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2016第二弹之细胞治疗中病毒载体的下游纯化
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近年来,以CAR-T为代表的的特异性细胞免疫治疗为攻克癌症带来了一丝曙光,越来越多的创业公司及科研单位纷纷投身于该领域,方兴未艾!在细胞治疗整个流程中涉及细胞分选,培养,质粒及病毒包装纯化等,高品质的病毒载体在CAR-T疗法中至关重要。病毒载体的纯化方式可分为:超滤法,超高速离心法以及层析法等。其中,层析法对设备要求低,具有时间短,通量高,纯度高和易于放大的特点,是一种非常重要的病毒载体纯化手段。在本次报告中,我们将讨论如何利用不同的层析介质以及合理的衔接步骤对病毒载体进行纯化。
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