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在2部分,普拉瑟介绍了她的实验室使用的设计原则设计E. coli从葡萄糖生产葡萄糖二酸。葡糖酸不在细菌中自然产生的所以,普拉瑟和她的同事们“bioprospected”从其他生物酶并在大肠杆菌中表达,构建所需的酶途径。普拉瑟走我们通过优化时序的许多步骤,酶表达的定位和水平,以产生最大的产量。
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