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Affymetix芯片原理--陈巍学基因(28)
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陈巍学基因这个节目,主要是为大家介绍基因组学,和临床分子诊断的最新技术进展.

今天,主要和大家谈一下著名的生物芯片公司 Affymetrix 公司,以及它应用得最广的两种芯片:表达谱芯片、和SNP分型芯片。主要分为以下几个部分:

1.Affymetrix 芯片的制造过程,类似半导体芯片的制造过程,是通过光蚀刻来完成的。

2.Affymetrix 的表达谱芯片,分成传统的In Vitro Transcription 芯片和Whole Transcriptome 芯片。

3.Affymetrix 公司的基因分型芯片,也就是SNP分型芯片有两种实验原理:新的是Axiom芯片,是基于连接反应的;而老的卡式芯片,是基于目标DNA片段与探针序列进行杂交。

4.Affymetrix 分析表达谱的软件。
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