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晶芯® 人类长链非编码RNA芯片V3.0的设计及在疾病研究中的应用
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长链非编码RNA (lncRNA)以RNA的形式在多种层面上,通过影响染色质状态,RNA转录和翻译层面调控基因的表达。近年研究者非常关注lncRNA在各种生物学过程和疾病过程中所起到的作用,形成了新的研究热点。 博奥生物与中科院生物物理所陈润生院士研究组,基于已经公布的大量lncRNA数据库及实验室发现的800多条中等长度的非编码RNA,推出了自主设计的lncRNA芯片。继成功推出lncRNA V1.0 和 V2.0 芯片基础上,鉴于当前lncRNA 研究的快速进展,收集和更新lncRNA 序列信息,经过严格的序列筛选和整合,推出了新一代的晶芯lncRNA V3.0 芯片服务。最新的V3.0芯片包括约3.8万条lncRNA和约3.4万条mRNA探针,可以同时针对lncRNA和mRNA进行检测。在充分保证探针容量和重复数量(lncRNA和mRNA检测探针均重复2次以上)的前提下,降低了芯片成本和实验费用。在检测成本和检测准确性之间达到较好的平衡点。 通过lncRNA芯片检测,研究人员能够迅速获得与特定生物学过程或者疾病相关的lncRNA的表达变化,从而发现与特定生物学过程相关的lncRNA,寻找与疾病相关的lncRNA。
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