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囊泡运输的生化重组
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Randy Schekman (Berkeley) Part 2: Biochemical Reconstitution of Transport Vesicle Budding

Secretion mutants that block protein exit from the endoplasmic reticulum define genes involved in the formation, targeting and fusion of a small vesicle intermediate. SEC genes corresponding to the mutants defective in vesicle budding define the cytoplasmic machinery responsible for transport vesicle morphogenesis. A biochemical reaction that reproduces ER vesicle budding was reconstituted with gently-broken yeast cells and pure recombinant Sec proteins required in vivo for this budding event. The Sec proteins assemble on the ER membrane in the presence of GTP which activates a small GTPase, Sar1, initiating the formation of a coat protein complex called COPII. See more athttp://www.ibioseminars.org
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  • 讲师:兰迪·谢克曼(化学家)

    兰迪·韦恩·谢克曼,美国细胞生物学家,加州大学伯克利分校教授,曾任《美国国家科学院院刊》主编。 1992年当选美国国家科学院院士。2002年与詹姆斯·罗思曼因对细胞膜传输的研究获拉斯克基础医学奖。2013年获得诺贝尔生理学或医学奖。