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课程>如何提高NGS样品制备实验成功率-详情
如何提高NGS样品制备实验成功率
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新一代测序技术过程中样品制备的质量是测序结果可靠性的根本保障，从核酸的提取、DNA 片段化、末端修复、标记添加，以及产物磁珠纯化、文库构建的每一个步骤，都对最终的实验结果有着巨大的影响。
除了拥有一台高大上的NGS测序仪以及配套的仪器和试剂是远远不够的。很多用户在实验操作中都被繁琐冗长的样品制备过程所困扰，从而严重影响了NGS实验的成功率。其实，从样本核酸的获得到文库构建，每一个步骤都对最终的实验结果产生失之毫厘谬以千里的影响。
在整个繁琐和复杂的NGS样本制备过程中，很多用户都有着诸如此类的疑问： 怎样减少可能出现的手工失误，如何选择合适实验工具进行，怎样避免样本的交叉污染，如何提高珍贵样品的文库构建效果，如何保证RNA样品的测序成功率？
在此次系列NGS网络讲座中，拥有丰富分子生物学产品的Eppendorf 公司将会为各位老师带来涉及NGS样本制备过程中的一系列技巧：
1. 影响文库构建成功率的操作因素；
2. 怎样避免样本制备过程中的交叉污染；
3. 如何保证微量样品文库构建的成功率；
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