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如何在斑马鱼完整胚胎样本中使用RNAscope®技术进行研究
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整胚原位杂交是在完整胚胎和组织中研究基因时空表达模式的有力工具。但现有的实验方法无法准确的直接检测RNA,而且操作耗时,结果和蛋白表达水平不一致。新一代原位定量杂交技术RNAscope®可以在斑马鱼整胚上实现快速高效、精准定量、特异性的多重RNA原位检测。该webinar由美国ACD公司(Advanced Cell Diagnostics, Inc., California, USA)邀请德国Münster大学细胞生物学研究所Erez Raz教授实验室两位研究员Azadeh Paksa 和 Theresa Gros介绍他们利用RNAscope®技术实现斑马鱼整胚上同时原位检测3个RNA分子进行三维荧光分析。详细介绍了实验操作过程,如何优化条件,降低信噪比以及RNAscope®技术相比传统原位杂交技术的绝对优势。

ACD公司提供RNAscope®原位定量杂交专利技术和产品,详细信息请访问ACD官网www.acdbio.com。更多中文资料请关注中国官方微信号(ACD_China)咨询。 文章题目: Simultaneous high-resolution detection of multiple transcripts combined with localization of proteins in whole-mount embryos. Gross-Thebing T, Paksa A, Raz E. BMC Biol. 2014 Aug 15;12(1):55.
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