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使用安捷伦 Seahorse XF 分析仪实时分析功能代谢表型以进行干细胞研究
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细胞能量代谢和干细胞功能调控之间存在明确的关联性。近期研究数据表明,需要将代谢表型从相对以糖酵解为主转变为相对以氧化磷酸化为主,以分化为特定细胞(例如神经细胞)。这个过程在细胞从相对增殖状态进入通常的分解代谢、已分化状态时发生。目前的研究工作主要集中在利用代谢技术手段调控细胞,从而促进特定细胞谱系分化。安捷伦 Seahorse XF 分析仪通过对两个重要细胞通路的速率进行定量,来测定代谢表型。安捷伦采用活细胞和无创无损的方式进行测定。目前,研究人员正在运用该技术确定细胞分化时的最优代谢表型。本演示文稿将介绍这些领域中的最新研究,以及在这些研究中用于评估相对代谢平衡的代谢表型分析的基本原理。
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