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细胞转染介绍
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课程介绍
转染是将遗传物质如DNA和双链RNA导入到哺乳动物细胞内的过程。将DNA导入细胞使得它可利用细胞自身的细胞机制表达和合成蛋白质,将RNA导入细胞则是用来通过终止翻译来降低某特定蛋白的合成。转染的RNA在细胞质中发挥作用,DNA则需被转运到细胞核中从而达到有效的转染。在核内,DNA会被短暂表达或整合到基因组DNA而将该遗传改变传代到下一代细胞。

本短片讲述了化学转染的基本原理,介绍了一些最常用的试剂,包括带电荷脂质体,聚合物和磷酸钙。我们将讲解从准备转染的细胞开始到最后分析转染效率过程中的每个步骤。另外,本视频文章的应用部分还讲述了导入核酸到哺乳动物细胞的其他方法,电击转染和基因枪转染。它还介绍了转染的更进一步的应用,同时转染DNA和干扰RNA来下调某天然存在的蛋白并同时引入该蛋白的突变体到同一细胞。
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