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谷峰:基因编辑的保真性与相关的应对策略
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课程介绍
介绍了CRISPR/Cas9作为最强大的基因编辑工具之一,目前最重要的缺点仍然包括脱靶问题,虽然有多个课题组从不同的角度去试图解决该重大科学问题。利用多种方法对Cas9编码蛋白基因进行操作,并利用特异性的靶向系统进行筛选,目前已经获得多个具有高保真CRISPR/Cas9的新型系统。展示了具有相关的研究成果。围绕基因编辑的保真性问题,腺相关病毒(AAV)介导的人类基因编辑目前是人类不切割基因组所获得的最高基因编辑的方法。但是其保真性如何并不清晰。
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